Coding

Part:BBa_K4061010:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-09-30)


Engineered OmpR (facilitates scaffolding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 772


Design Notes

The linker was chosen as GS chain since we wanted an inert chain that does not interact with our protein. We tested this by running the protein sequence in Swiss Model.


Source

OmpR sequence taken from UniProt database, genome of E. coli 1.2741. The Leucine zipper is inspired from "Engineering robust control of two-component system phosphotransfer using modular scaffolds".Whitaker, W. R. et al.2012. PNAS

References